The Ultimate Guide To high performance liquid chromatography
The Ultimate Guide To high performance liquid chromatography
Blog Article
I would really like to enroll in newsletters from Sartorius (Sartorius AG and its affiliated businesses) primarily based of my individual interests.
The column size is similar. The column is full of silica particles which are modified for making them non-polar. This really is carried out by attaching very long hydrocarbon chains (eight–18 C atoms) to its surface.
The realm of the peak is immediately detected by the computer. The computer also detect the retention time of that certain element.
The cellular period would be the solvent combination that repeatedly flows throughout the HPLC system, carrying the sample from the column. It performs an important purpose in separating the analytes:
物質にエネルギーを与える（励起）ことにより発光する（蛍光）性質を利用した検出器。一般に選択性が高く高感度で、物質に特異的な検出が可能。蛍光する性質を持たない物質については、その物質を標識することにより検出が可能になる。
-hydroxybenzoic acid—on a nonpolar C18 column working with an aqueous buffer of acetic acid and sodium acetate as the cellular section. The retention periods for these weak acids are shorter when using a a lot less acidic mobile phase because each solute is existing within an anionic, weak base sort that may be a lot less soluble while in the nonpolar stationary section.
The solvent reservoir keep the solvent or mobile period to provide towards the column as necessary. The solvent is pumped to the column in a particular circulation price.
, for example, has two cell period reservoirs that happen high performance liquid chromatography to be utilized for an isocratic elution or perhaps a gradient elution by drawing solvents from just one or each reservoirs.
The data acquisition system information and procedures the alerts in the detector, permitting for the development of chromatograms as well as high performance liquid chromatography the quantification of compounds.
A pump forces a solvent via a column beneath high pressures of as many as 400 atmospheres. The column packing product or adsorbent or stationary stage is typically a granular material of reliable particles for example silica or polymers.
The stationary section is usually a sound aid packed inside a column, Whilst the cellular period is normally a liquid or a combination of liquids.
The area underneath Each individual peak is proportional to the amount of the corresponding analyte. The information acquisition system permits the Examination of peak retention occasions, peak places, along with the calculation of analyte concentrations.
ノブをインジェクト側に切り替え、サンプルを流路に注入する。マニュアルインジェクターに電気信号を出力する機能が付いていれば、この時にインジェクション信号を検出器またはインテグレーターに送ることが出来る。
A quantitative HPLC Assessment is often simpler than the usual quantitative GC Investigation due to the fact a set volume sample loop provides a more specific and exact injection.